Tag
6xHisB5-B5

Part:BBa_K2984014:Design

Designed by: Sophia Schiebler   Group: iGEM19_Humboldt_Berlin   (2019-10-14)


6x His-Tag for protein detection, isolation, purification B5-B5


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was translated to base triplets, codon-optimized for C. reinhardtii


Source

Sugiura, M., Inoue, Y., & Minagawa, J. (1998). Rapid and discrete isolation of oxygen-evolving His-tagged photosystem II core complex from Chlamydomonas reinhardtii by Ni2+ affinity column chromatography. FEBS Lett, 426(1), 140-144.

References